首页 > 研究成果 > 2021年

  1. Aging Atlas: a multi-omics database for aging biology. Nucleic Acids Res, 2021. 49: D825-d830.
  2. Bi D#, Yao J#, Wang Y, Qin G, Zhang Y, Wang Y, Zhao J*. CRISPR/Cas13d-mediated efficient KDM5B mRNA knockdown in porcine somatic cells and parthenogenetic embryos. Reproduction, 2021. 162: 149-160.
  3. Bian X#, Liu J, Yang Q, Liu Y, Jia W, Zhang X, Li YX, Shao X*, Wang YL*. MicroRNA-210 regulates placental adaptation to maternal hypoxic stress during pregnancy. Biol Reprod, 2021. 104: 418-429.
  4. Cao J#, Hao J#, Wang L#, Tan Y, Tian Y, Li S, Ma A, Fu B, Dai J, Zhai P, Xiang P, Zhang Y, Cheng T*, Peng Y*, Zhou Q*, Zhao T*. Developing standards to support the clinical translation of stem cells. Stem Cells Transl Med, 2021. 10 Suppl 2: S85-s95.
  5. Chen L#, Gao H#, Zhou B*, Wang Y*. Comprehensive optimization of a reporter assay toolbox for three distinct CRISPR-Cas systems. FEBS Open Bio, 2021a. 11: 1965-1980.
  6. Chen M#, Dong F#, Chen M, Shen Z, Wu H, Cen C, Cui X, Bao S, Gao F*. PRMT5 regulates ovarian follicle development by facilitating Wt1 translation. Elife, 2021b. 10.
  7. Chen Y#, Liu C, Shang Y, Wang L, Li W*, Li G*. Adam21 is dispensable for reproductive processes in mice. PeerJ, 2021c. 9: e12210.
  8. Cui D#, Liu Y#, Jiang X#, Ding C, Poon LC*, Wang H*, Yang H*. Single-cell RNA expression profiling of SARS-CoV-2-related ACE2 and TMPRSS2 in human trophectoderm and placenta. Ultrasound Obstet Gynecol, 2021. 57: 248-256.
  9. Dai SK#, Liu PP, Du HZ, Liu X, Xu YJ, Liu C, Wang YY, Teng ZQ*, Liu CM*. Histone crotonylation regulates neural stem cell fate decisions by activating bivalent promoters. EMBO Rep, 2021. 22: e52023.
  10. Diao Z#, Ji Q#, Wu Z#, Zhang W#, Cai Y, Wang Z, Hu J, Liu Z, Wang Q, Bi S, Huang D, Ji Z, Liu GH*, Wang S*, Song M*, Qu J*. SIRT3 consolidates heterochromatin and counteracts senescence. Nucleic Acids Res, 2021. 49: 4203-4219.
  11. Dong F#, Chen M#, Chen M, Jiang L, Shen Z, Ma L, Han C*, Guo X*, Gao F*. PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Plzf Expression via Modulation of Lysine Histone Modifications. Front Cell Dev Biol, 2021. 9: 673258.
  12. Du X#, Guo Z#, Fan W#, Hai T#, Gao F, Li P, Qin Y, Chen C, Han Z, Ren J, Jiao P, Liu W, Bi Y*, Yu D*, Wu S*. Establishment of a humanized swine model for COVID-19. Cell Discov, 2021. 7: 70.
  13. Gu P#, Hui X#, Zheng Q#, Gao Y, Jin L, Jiang W, Zhou C, Liu T, Huang Y, Liu Q, Nie T, Wang Y, Wang Y, Zhao J*, Xu A*. Mitochondrial uncoupling protein 1 antagonizes atherosclerosis by blocking NLRP3 inflammasome-dependent interleukin-1β production. Sci Adv, 2021. 7: eabl4024.
  14. Guo Y#, Xu B#, Wu Z, Bo J, Tong C, Chen D, Wang J, Wang H*, Wang Y*, Han W*. Mutant B2M-HLA-E and B2M-HLA-G fusion proteins protects universal chimeric antigen receptor-modified T cells from allogeneic NK cell-mediated lysis. Eur J Immunol, 2021. 51: 2513-2521.
  15. Ji WH#, Li DD#, Wei DP, Gu AQ, Yang Y, Peng JP*. Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period. Front Immunol, 2021. 12: 763067.
  16. Jin L#, Cao L#, Zhu Y#, Cao J#, Li X, Zhou J, Liu B, Zhao T*. Enhance anti-lung tumor efficacy of chimeric antigen receptor-T cells by ectopic expression of C–C motif chemokine receptor 6. Science Bulletin, 2021. 66: 803-812.
  17. Khawar MB#, Liu C#, Gao F#, Gao H, Liu W, Han T, Wang L, Li G*, Jiang H*, Li W*. Sirt1 regulates testosterone biosynthesis in Leydig cells via modulating autophagy. Protein Cell, 2021. 12: 67-75.
  18. Lei J#, Wang S#, Kang W#, Chu Q, Liu Z, Sun L, Ji Y, Esteban CR, Yao Y, Belmonte JCI, Chan P, Liu GH*, Zhang W*, Song M*, Qu J*. FOXO3-engineered human mesenchymal progenitor cells efficiently promote cardiac repair after myocardial infarction. Protein Cell, 2021a. 12: 145-151.
  19. Lei WL#, Li YY#, Meng TG#, Ning Y, Sun SM, Zhang CH, Gui Y, Wang ZB*, Qian WP*, Sun QY*. Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis. Cell Death Dis, 2021b. 12: 883.
  20. Li CW#, Yu K#, Shyh‐Chang N#, Li GX, Yu SL, Liu HJ, Yang B, Li ZY, Zhao YJ, Xu LY*. Sterol metabolism and protein metabolism are differentially correlated with sarcopenia in Asian Chinese men and women. Cell proliferation, 2021a. 54: e12989.
  21. Li D#, Li F#, Meng L, Wei H, Zhang Q, Jiang F, Chen DN, Li W*, Tan YQ*, Li JD*. RNF216 regulates meiosis and PKA stability in the testes. Faseb j, 2021b. 35: e21460.
  22. Li J#, Zheng Y#, Yan P#, Song M#, Wang S#, Sun L, Liu Z, Ma S, Izpisua Belmonte JC, Chan P, Zhou Q, Zhang W*, Liu GH*, Tang F*, Qu J*. A single-cell transcriptomic atlas of primate pancreatic islet aging. Natl Sci Rev, 2021c. 8: nwaa127.
  23. Li SY#, Liu Y#, Wang L#, Wang F, Zhao TT, Li QY, Xu HW, Meng XH, Hao J, Zhou Q, Wang L*, Yin ZQ*. A phase I clinical trial of human embryonic stem cell-derived retinal pigment epithelial cells for early-stage Stargardt macular degeneration: 5-years' follow-up. Cell Prolif, 2021d. 54: e13100.
  24. Li X#, Wang Y#, Ma R#, Liu X, Song B, Duan Y, Guo J, Feng G, Cui T, Wang L Hao J*, Wang H*, Gu Q*. Reconstruction of functional uterine tissues through recellularizing the decellularized rat uterine scaffolds by MSCs in vivo and in vitro. Biomedical Materials, 2021e. 16: 035023.
  25. Li YY#, Guo L#, Li H#, Lei WL#, Fan LH, Ouyang YC, Hou Y, Wang ZB*, Sun QY*, Lu SS*, Han Z*. PTHrP promotes development of mouse preimplantation embryos through the AKT/cyclin D1 pathway and nuclear translocation of HDAC4. J Cell Physiol, 2021f. 236: 7001-7013.
  26. Liang C#, Liu Z#, Song M#, Li W, Wu Z, Wang Z, Wang Q, Wang S, Yan K, Sun L, Hishida T, Cai Y, Belmonte JCI, Guillen P, Chan P, Zhou Q, Zhang W*, Qu J*, Liu GH*. Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration. Cell Res, 2021a. 31: 187-205.
  27. Liang G#, Zhou C#, Jiang X#, Zhang Y#, Huang B, Gao S, Kang Z, Ma D, Wang F, Gottgens B, Wang H*, Han JJ*, Liu F*. De novo generation of macrophage from placenta-derived hemogenic endothelium. Dev Cell, 2021b. 56: 2121-2133.e2126.
  28. Liu C#, Dai SK#, Shi RX, He XC, Wang YY, He BD, Sun XW, Du HZ, Liu CM*, Teng ZQ*. Transcriptional profiling of microglia in the injured brain reveals distinct molecular features underlying neurodegeneration. Glia, 2021a. 69: 1292-1306.
  29. Liu C#, Liu H#, Zhang H, Wang L, Li M, Cai F, Wang X, Wang L, Zhang R, Yang S, Liu W, Liang Y, Wang L, Song X, Su S, Gao H, Jiang J, Li J, Luo M, Gao F, Chen Q, Li W*, Chen ZJ*. Paternal USP26 mutations raise Klinefelter syndrome risk in the offspring of mice and humans. Embo j, 2021b. 40: e106864.
  30. Liu J#, Hou Z#, Wu J#, Liu K, Li D, Gao T, Liu W, An B, Sun Y, Mo F, Wang L, Wang Y*, Hao J*, Hu B*. Infusion of hESC derived Immunity‐and‐matrix regulatory cells improves cognitive ability in early‐stage AD mice. Cell proliferation, 2021c. 54: e13085.
  31. Liu J#, Shao X#, Qin W#, Zhang Y#, Dang F, Yang Q, Yu X, Li YX, Chen X*, Wang C*, Wang YL*. Quantitative chemoproteomics reveals O-GlcNAcylation of cystathionine γ-lyase (CSE) represses trophoblast syncytialization. Cell Chem Biol, 2021d. 28: 788-801.e785.
  32. Liu X#, Dai SK, Liu PP, Liu CM*. Arid1a regulates neural stem/progenitor cell proliferation and differentiation during cortical development. Cell proliferation, 2021e. 54: e13124.
  33. Lu X#, Zhang Y#, Wang L#, Wang L#, Wang H, Xu Q, Xiang Y, Chen C, Kong F, Xia W, Lin Z, Ma S, Liu L, Wang X, Ni H, Li W*, Guo Y*, Xie W*. Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting. Sci Adv, 2021. 7: eabi6178.
  34. Ma H#, Su L#, Xia W, Wang W, Tan G*, Jiao J*. MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors. EMBO Rep, 2021a. 22: e52150.
  35. Ma S#, Sun S#, Li J#, Fan Y#, Qu J#, Sun L, Wang S, Zhang Y, Yang S, Liu Z, Wu Z, Zhang S, Wang Q, Zheng A, Duo S, Yu Y, Belmonte JCI, Chan P, Zhou Q, Song M*, Zhang W*, Liu GH*. Single-cell transcriptomic atlas of primate cardiopulmonary aging. Cell Res, 2021b. 31: 415-432.
  36. Ma Y#, Yu X#, Li YX, Wang YL*. HGF/c-Met signaling regulates early differentiation of placental trophoblast cells. J Reprod Dev, 2021c. 67: 89-97.
  37. Ma Y#, Yu X#, Zhang L#, Liu J, Shao X, Li YX, Wang YL*. Uterine decidual niche modulates the progressive dedifferentiation of spiral artery vascular smooth muscle cells during human pregnancy. Biol Reprod, 2021d. 104: 624-637.
  38. Mao Y#, Wang L#, Zhong B#, Yang N#, Li Z, Cui T, Feng G, Li W, Zhang Y*, Zhou Q*. Continuous expression of reprogramming factors induces and maintains mouse pluripotency without specific growth factors and signaling inhibitors. Cell proliferation, 2021. 54: e13090.
  39. Nan X#, Zhang B#, Hao J#, Yue W#, Fu B, Qu M, Zhang Y, Wang H, Fang F, Wei J, Li Q, Hu S, Yu J, Gao Y, Liu Q, Cao J, Wang L, Peng Y, Zhu H, Liang L, Ma A*, Zhou J*, Zhao T*, Pei X*. Requirements for human haematopoietic stem/progenitor cells. Cell proliferation, 2022. 55(4):e13152
  40. Peng C#, Lv Z#, Hai T, Dai X*, Zhou Q*. Differential effects of trichostatin A on mouse embryogenesis and development. Reproduction, 2021. 162: 83-94.
  41. Peng Z#, Wu J#, Hu S#, Ma A#, Wang L#, Cao N, Zhang Y, Li Q, Yu J, Meng S, Na T, Shi X, Li M, Liu H, Qian L, Tian E, Lin F, Cao J, Peng Y, Zhu X, Liang L, Hao J, Zhao T*, Cheng X*, Pan G*. Requirments for primary human hepatocyte. Cell proliferation, 2022. 55: e13147.
  42. Qu Y#, Chen Q#, Guo S#, Ma C, Lu Y, Shi J, Liu S, Zhou T, Noda T, Qian J, Zhang L, Zhu X, Lei X, Cao Y, Li W, Li W, Plachta N, Matzuk MM, Ikawa M, Duan E*, Zhang Y*, Wang H*. Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization. Protein Cell, 2021. 12: 810-817.
  43. Shao X#, Cao G#, Chen D#, Liu J, Yu B, Liu M, Li YX, Cao B*, Sadovsky Y*, Wang YL*. Placental trophoblast syncytialization potentiates macropinocytosis via mTOR signaling to adapt to reduced amino acid supply. Proc Natl Acad Sci U S A, 2021. 118.
  44. Shi J#, Ma Y#, Hua H, Liu Y, Li W*, Yu H*, Liu C*. Dynamic Histone H3 Modifications Regulate Meiosis Initiation via Respiration. Frontiers in Cell and Developmental Biology, 2021. 9: 646214.
  45. Shyh-Chang N*, Li L*. Stabilizing Formative Pluripotent States with Germ Cell Competency. Cell Stem Cell, 2021. 28: 361-363.
  46. Song R#, Liu K#, Wang Y, Qin G, Xiao M, Liu Y, Yang J, Zong R, Yan X, Zheng Q, Zhang H, Hou N, Yao J, Wang X, Wang Y, Zhao J*, Zhang X*. A pig model carrying heterozygous point mutation of NCSTN simulates familial acne inversa and reveals dysregulated cholesterol biosynthesis via the Notch-pAMPK-HMGCR pathway. Science Bulletin, 2021. 66: 2343-2346.
  47. Su R#, Fan LH#, Cao C#, Wang L, Du Z, Cai Z, Ouyang YC, Wang Y, Zhou Q, Wu L, Zhang N, Zhu X, Lei WL, Zhao H, Tian Y, He S, Wong CCL*, Sun QY*, Xue Y*. Global profiling of RNA-binding protein target sites by LACE-seq. Nat Cell Biol, 2021. 23: 664-675.
  48. Sun Y#, Feng L#, Liang L#, Stacey GN, Wang C, Wang Y*, Hu B*. Neuronal cell-based medicines from pluripotent stem cells: Development, production, and preclinical assessment. Stem Cells Transl Med, 2021. 10 Suppl 2: S31-s40.
  49. Wang C#, Liu K#, Cao J#, Wang L, Zhao Q, Li Z, Zhang H, Chen Q, Zhao T*. PINK1‐mediated mitophagy maintains pluripotency through optineurin. Cell proliferation, 2021a. 54: e13034.
  50. Wang F#, Jia W#, Fan M#, Shao X#, Li Z, Liu Y, Ma Y, Li YX, Li R, Tu Q*, Wang YL*. Single-cell Immune Landscape of Human Recurrent Miscarriage. Genomics Proteomics Bioinformatics, 2021b. 19: 208-222.
  51. Wang L#, Cao J#, Peng Y#, Fu B#, Jin Z, Hu Y, Wu W, Xiang AP, Hu S, Yu J, Zhang Y, Wei J, Zhang Y, Li Q, Zhou J, Zhai P, Zhu H, Liang L, Ma A*, Glyn Stacey*, Zhao T*, Hao J*. Human retinal pigment epithelial cells. Cell proliferation, 2022. 55: e13153.
  52. Wang L#, Xu Z#, Wang L#, Liu C#, Wei H, Zhang R, Chen Y, Wang L, Liu W, Xiao S, Li W*, Li W*. Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming. Cell Prolif, 2021c. 54: e13080.
  53. Wang S#, Yao X#, Ma S#, Ping Y#, Fan Y, Sun S, He Z, Shi Y, Sun L, Xiao S, Song M, Cai J, Li J, Tang R, Zhao L, Wang C, Wang Q, Zhao L, Hu H, Liu X, Sun G, Chen L, Pan G, Chen H, Li Q, Zhang P, Xu Y, Feng H, Zhao GG, Wen T, Yang Y, Huang X, Li W, Liu Z, Wang H, Wu H, Hu B, Ren Y, Zhou Q, Qu J*, Zhang W*, Liu GH*, Bian XW*. A single-cell transcriptomic landscape of the lungs of patients with COVID-19. Nat Cell Biol, 2021d. 23: 1314-1328.
  54. Wang W#, Zheng Y#, Sun S#, Li W#, Song M, Ji Q, Wu Z, Liu Z, Fan Y, Liu F, Li J, Esteban CR, Wang S, Zhou Q, Belmonte JCI, Zhang W*, Qu J*, Tang F*, Liu GH*. A genome-wide CRISPR-based screen identifies KAT7 as a driver of cellular senescence. Sci Transl Med, 2021e. 13.
  55. Wang X#, Xiang Y#, Yu Y#, Wang R, Zhang Y, Xu Q, Sun H, Zhao ZA, Jiang X, Wang X, Lu X, Qin D, Quan Y, Zhang J, Shyh-Chang N, Wang H, Jing N, Xie W*, Li L*. Formative pluripotent stem cells show features of epiblast cells poised for gastrulation. Cell Res, 2021f. 31: 526-541.
  56. Wang Z#, Li N#, Feng K#, Chen M, Zhang Y, Liu Y, Yang Q, Nie J, Tang N, Zhang X, Cheng C, Shen L, He J, Ye X, Cao W*, Wang H*, Han W*. Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors. Cell Mol Immunol, 2021g. 18: 2188-2198.
  57. Wei DP#, Li DD#, Gu AQ, Ji WH, Yang Y, Peng JP*. A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface. J Cell Mol Med, 2021. 25: 1771-1782.
  58. Wu B#, Yu X, Liu C, Wang L, Huang T, Lu G, Chen ZJ*, Li W*, Liu H*. Essential Role of CFAP53 in Sperm Flagellum Biogenesis. Front Cell Dev Biol, 2021a. 9: 676910.
  59. Wu H#, Liao S#, Wang Y#, Guo M#, Lin X, Wu J, Wang R, Lv D, Wu D, He M, Long R, Peng J, Yang H, Yin H, Wang X, Huang Z, Lan K, Zhou Y, Zhang W, Xiao Z, Zhao Y*, Deng D*, Wang H*. Molecular evidence suggesting the persistence of residual SARS‐CoV‐2 and immune responses in the placentas of pregnant patients recovered from COVID‐19. Cell proliferation, 2021b. 54: e13091.
  60. Xing D#, Wang K#, Wu J#, Zhao Y, Liu W, Li JJ, Gao T, Yan D, Wang L, Hao J*, Lin J*. Clinical-Grade Human Embryonic Stem Cell-Derived Mesenchymal Stromal Cells Ameliorate the Progression of Osteoarthritis in a Rat Model. Molecules, 2021. 26.
  61. Yan R#, Cheng X#, Guo F*. Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells. STAR Protoc, 2021a. 2: 100972.
  62. Yan R#, Gu C#, You D#, Huang Z, Qian J, Yang Q, Cheng X, Zhang L, Wang H*, Wang P*, Guo F*. Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing. Cell Stem Cell, 2021b. 28: 1641-1656.e1647.
  63. Yang Q#, Ma Y#, Liu Y, Shao X, Jia W, Yu X, Li Yx, Yang L, Gu W, Wang H, Wang J*, Wang Y*. MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness. Cell proliferation, 2021a. 54: e13145.
  64. Yang R#, Liu C#, Li Q, Wang W, Wu B, Chen A, Wang B*, Li W*, Chen J. Artificial intelligence based identification of the functional role of hirudin in diabetic erectile dysfunction treatment. Pharmacol Res, 2021b. 163: 105244.
  65. Yao J#, Wang Y#, Cao C#, Song R, Bi D, Zhang H, Li Y, Qin G, Hou N, Zhang N, Zhang J, Guo W, Yang S, Wang Y, Zhao J*. CRISPR/Cas9-mediated correction of MITF homozygous point mutation in a Waardenburg syndrome 2A pig model. Mol Ther Nucleic Acids, 2021. 24: 986-999.
  66. Yu M#, Lei W#, Cao J#, Wang L#, Ma A, Zhao ZA, Yang HT, Shen Z, Lan F, Cao F, Liang P, Pei X, Xiang P, Yu J, Zhang Y, Zhang Y, Li Q, Zhou J, Wei J, Peng Y, Zhu H, Liang L, Cao N*, Fu B*, Hao J*, Zhao T*, Hu S*. Requirements for human cardiomyocytes. Cell proliferation, 2022. e13150.
  67. Zhang H#, Li J#, Ren J#, Sun S, Ma S, Zhang W, Yu Y, Cai Y, Yan K, Li W, Hu B, Chan P, Zhao GG, Belmonte JCI, Zhou Q, Qu J*, Wang S*, Liu GH*. Single-nucleus transcriptomic landscape of primate hippocampal aging. Protein Cell, 2021a. 12: 695-716.
  68. Zhang J#, Ma Y-X#, Zeng Y-Q, Zhang S-F, Teng Z-Q, Gao J*, Liu C-M*. miR-26a promotes axon regeneration in the mammalian central nervous system by suppressing PTEN expression. Acta Biochimica et Biophysica Sinica, 2021b. 53: 758-765.
  69. Zhang Y#, Liu C#, Wu B, Li L, Li W*, Yuan L*. The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus. Nat Commun, 2021c. 12: 4926.
  70. Zhao BW#, Sun SM, Xu K, Li YY, Lei WL, Li L, Liu SL, Ouyang YC, Sun QY*, Wang ZB*. FBXO34 Regulates the G2/M Transition and Anaphase Entry in Meiotic Oocytes. Front Cell Dev Biol, 2021. 9: 647103.
  71. Zhou S#, Guo Y, Sun H, Liu L, Yao L, Liu C, He Y, Cao S, Zhou C, Li M, Cao Y, Wang C, Lu Q, Li W*, Guo X*, Huo R*. Maternal RNF114-mediated target substrate degradation regulates zygotic genome activation in mouse embryos. Development, 2021. 148.
  72. Zhu F-Y#, Wang L-L#, Meng T-G#, Wang R-L#, Yang Z-X#, Cao Y, Zhu G-Y, Jin Z, Gao L-L, Zeng W-T, Wang Z*, Sun Q*, Zhang D*. Inhibiting bridge integrator 2 phosphorylation leads to improved oocyte quality, ovarian health and fertility in aging and after chemotherapy in mice. Nature Aging, 2021a. 1: 1010-1023.
  73. Zhu H#, Sun H#, Yu D, Li T, Hai T, Liu C, Zhang Y, Chen Y, Dai X, Li Z, Li W, Liu R*, Feng G*, Zhou Q*. Transcriptome and DNA Methylation Profiles of Mouse Fetus and Placenta Generated by Round Spermatid Injection. Front Cell Dev Biol, 2021b. 9: 632183.
  74. Zou Z#, Long X#, Zhao Q#, Zheng Y#, Song M, Ma S, Jing Y, Wang S, He Y, Esteban CR, Yu N, Huang J, Chan P, Chen T, Izpisua Belmonte JC*, Zhang W*, Qu J*, Liu GH*. A Single-Cell Transcriptomic Atlas of Human Skin Aging. Dev Cell, 2021. 56: 383-397.e388.