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郭帆 研究员

生殖表观遗传研究

欢迎您的加入生殖表观遗传研究组!
- 工作人员:曹羽、侯昕伶
- 博士后:钱静静
- 研究生:赵东红、龙鑫、燕蕊、张瑞丰、程馨、郭敬、张前程、孙奉圆、张帆、朱然
- 本科生:梁佳琪

 

 

  表观遗传调控在哺乳动物发育过程中至关重要,其对基因转录活性、染色质状态以及高级结构的改变将影响多能性的建立与维持、细胞命运决定与谱系分化、细胞稳态维持与功能等。虽然多种类型的表观遗传修饰(如DNA修饰、组蛋白修饰)及其相关调节因子(如修饰酶、去修饰酶)已被报道,但其在生理状态下的动态与功能仍有待深入研究。


图1 单个细胞不同分子层面的代表性组学方法。



图2 利用单细胞多组学方法解析人和小鼠卵细胞DNA甲基化的建立。



图3 肥胖影响卵泡发生的机理研究

 

 

研究内容和目标:
我们实验室将利用前沿的分子生物学方法、成像工具和在体模型等来研究:1. 生殖细胞发生与胚胎发育中的表观遗传编程和重编程;2. 不同表观遗传修饰之间的互作对细胞稳态与功能的影响;3. 细胞命运决定与转变过程中的表观遗传调控。

 

代表性发表论文:

  1. Qian, J. and F. Guo* (2022). "De novo programming: establishment of epigenome in mammalian oocytes." Biol Reprod.Online
  2. Yan, R., C. Gu*, D. You, Z. Huang, J. Qian, Q. Yang, X. Cheng, L. Zhang, H. Wang, P. Wang* and F. Guo* (2021). "Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing." Cell Stem Cell  28(9): 1641-1656.
  3. Long, X., Q. Yang, J. Qian, H. Yao, R. Yan, X. Cheng, Q. Zhang, C. Gu, F. Gao, H. Wang, L. Zhang* and F. Guo* (2021). "Obesity modulates cell-cell interactions during ovarian folliculogenesis." iScience  25(1): 103627.
  4. Yang, Z., X. Xu, C. Gu, J. Li, Q. Wu, C. Ye, A. V. Nielsen, L. Mao, J. Ye, K. Bai, F. Guo*, C. Tang* and Y. Zhao* (2020). "Chemicals orchestrate reprogramming with hierarchical activation of master transcription factors primed by endogenous Sox17 activation." Communications  Biology  3(1): 629.
  5. Zhou, C., Y. Sun, R. Yan, Y. Liu, E. Zuo, C. Gu, L. Han, Y. Wei, X. Hu, R. Zeng, Y. Li*, H. Zhou*, F. Guo* and H. Yang* (2019). "Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis." Nature  571(7764): 275-278.
  6. Gu, C., S. Liu, Q. Wu, L. Zhang and F. Guo* (2019). "Integrative single-cell analysis of transcriptome, DNA methylome and chromatin accessibility in mouse oocytes." Cell Research  29(2): 110-123. Cover story
  7. Chen, X., J. Zhao, C. Gu, Y. Cui, Y. Dai, G. Song, H. Liu, H. Shen, Y. Liu, Y. Wang, H. Xing, X. Zhu, P. Hao, F. Guo* and X. Liu* (2018). "Med23 serves as a gatekeeper of the myeloid potential of hematopoietic stem cells." Nature Communications  9(1): 3746.
  8. Li, L., F. Guo, Y. Gao, Y. Ren, P. Yuan, L. Yan, R. Li, Y. Lian, J. Li, B. Hu, J. Gao, L. Wen, F. Tang and J. Qiao (2018). "Single-cell multi-omics sequencing of human early embryos." Nature Cell Biology  20(7): 847-858.
  9. Guo, F.*, L. Li, J. Li, X. Wu, B. Hu, P. Zhu, L. Wen and F. Tang* (2017). "Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells." Cell Res earch  27(8): 967-988. Cover story
  10. Guo, F., L. Yan, H. Guo, L. Li, B. Hu, Y. Zhao, J. Yong, Y. Hu, X. Wang, Y. Wei, W. Wang, R. Li, J. Yan, X. Zhi, Y. Zhang, H. Jin, W. Zhang, Y. Hou, P. Zhu, J. Li, L. Zhang, S. Liu, Y. Ren, X. Zhu, L. Wen, Y. Q. Gao, F. Tang and J. Qiao (2015). "The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells." Cell  161(6): 1437-1452. Cover story
  11. Guo, F., X. Li, D. Liang, T. Li, P. Zhu, H. Guo, X. Wu, L. Wen, T. P. Gu, B. Hu, C. P. Walsh, J. Li, F. Tang and G. L. Xu (2014). "Active and passive demethylation of male and female pronuclear DNA in the mammalian zygote." Cell Stem Cell  15(4): 447-459.
  12. Gu, T. P., F. Guo, H. Yang, H. P. Wu, G. F. Xu, W. Liu, Z. G. Xie, L. Shi, X. He, S. G. Jin, K. Iqbal, Y. G. Shi, Z. Deng, P. E. Szabo, G. P. Pfeifer, J. Li and G. L. Xu (2011). "The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes." Nature  477(7366): 606-610.

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